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 Multiple Choice QuestionsMultiple Choice Questions

81.

cDNA probes are copied from the messenger RNA molecules with the help of

  • restriction enzymes

  • reverse transcriptase

  • DNA polymerase

  • adenosine deaminase


82.

Assertion : In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).

Reason : Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.

  • If both Assertion and Reason are true and the Reason is the correct explanation of the Assertion

  • If both Assertion and Reason are true but the Reason is not the correct explanation of the assertion

  • If Assertion is true but Reason is false.

  • If both Assertion and Reason are false


83.

What is the first step in the Southern Blot technique

  • Denaturation of DNA on the gel for hybridization with specific probe

  • Production of a group of genetically identical cells

  • Digestion of DNA by restriction enzyme

  • Isolation of DNA from a nucleated cell such as the one from the scene of crime


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84.

Restriction enzymes:

  • are endonucleases which cleave DNA at specific sites

  • make DNA complementary to an existing DNA or RNA

  • cut or join DNA fragments

  • are required in vectorless direct gene transfer.


A.

are endonucleases which cleave DNA at specific sites

Restriction enzymes are endonucleases which cleave DNA at specific sites. They are extracted from the bacterium E.coli.

Restriction enzymes Type I bind to a recognition site of a duplex DNA and cleave one strand only. Restriction Enzyme Type II are more valuable in gene manipulation and cleave the duplex at specific target sites at or near the binding site.


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85.

Which of the following enzymes is used to join bits of DNA?

  • Ligase

  • Primase

  • Endonuclease

  • DNA polymerase


86.

Polymerase chain reaction is most useful in

  • DNA synthesis

  • DNA amplification

  • protein synthesis

  • amino acid synthesis


87.

Shorter generation time of E. coli compared to eukaryotes may be explained on the basis of

  • shape

  • large surface : volume ratio

  • presence of cell wall

  • absence of organelles


88.

Which one among the following is just a cloning plasmid not an expression plasmid?

  • pBAD-18-Cam

  • pBCSK

  • pUC18

  • pET


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89.

Restriction enzymes are used to cut

  • single stranded RNA

  • double stranded DNA

  • single stranded DNA

  • double stranded RNA


90.

Molecular scissors, which cut DNA at specific site is

  • pectinase

  • polymerase

  • restriction endonuclease

  • ligase


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