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 Multiple Choice QuestionsMultiple Choice Questions

101.

Which of these statements is incorrect?

  • Enzymes of TCA cycle are present in mitochondrial matrix

  • Glycolysis occurs in cytosol

  • Oxidative phosphorylation takes place in outer mitochondrial membrane

  • Glycolysis operates as long as it is supplied with NAD that can pick up hydrogen atoms


C.

Oxidative phosphorylation takes place in outer mitochondrial membrane

Oxidative phosphorylation takes place in inner mitochondrial membrane.


102.

Paul berg's gene splicing experiment created the first rDNA molecule, which was a

  • T4 phage fragment incorporated into SV40 vector

  • Lambda phage fragment incorporated into SV40 vector

  • T4 phage fragment incorporated into pSC 101 vector

  • Lambda phage fragment incorporated into pSC 101 vector


B.

Lambda phage fragment incorporated into SV40 vector

Paul Berg's gene splicing experiment created the first rDNA molecule, which was a lambda phage fragment incorporated into SV40 vector.


103.

All monerons

  • Contain DNA and RNA

  • Demonstrate a long circular strand of DNA, not formed enclosed in a nuclear membrane

  • Are bacteria

  • All of the above


D.

All of the above


104.

The sequence given below 5'-GGCC-3' is recognition sequence for which of the following enzyme

  • Not 1

  • Eco R1

  • Hae III

  • Puv I


C.

Hae III

Hae III is extracted from Haemophiles influenzae it cuts G-C in 5'-3' direction in both templates.


105.

Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called

  • Bio-infringement

  • Biopiracy

  • Bioexploitation

  • Biodegradation


B.

Biopiracy

Biopiracy is a term used for or refers to the use of bioresources by multinational companies and other organisation without proper authorisation from the countries and people concerned with compensatory payment.


106.

In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is

  • Indian Council of Medical Research (ICMR)

  • Council for Scientific and Industrial Research (CSIR)

  • Genetic Engineering Appraisal Committee (GEAC)

  • Research Committee on Genetic Manipulation (RCGM)


C.

Genetic Engineering Appraisal Committee (GEAC)

Indian Government has setup organisation such as GEAC (Genetic Engineering Appraisal Committee) which will make decisions regarding the validity of GM research and safety of introducing GM-organism for public services.


107.

The DNA fragments separated on an agarose gel can be visualised after staining with

  • Bromophenol blue

  • Acetocarmine

  • Aniline blue

  • Aniline blue


D.

Aniline blue

Ethidium bromide is used to stain the DNA fragments and will appear as orange coloured bands under UV light.

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108.

A gene whose expression helps to identify transformed cell is known as

  • Selectable marker

  • Vector

  • Plasmid

  • Plasmid


A.

Selectable marker

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109.

The correct order of steps in Polymerase Chain Reaction (PCR) is

  • Extension, Denaturation, Annealing

  • Annealing, Extension, Denaturation

  • Denaturation, Annealing, Extension

  • Denaturation, Extension, Annealing


C.

Denaturation, Annealing, Extension

PCR is based on three steps:
(i) Denaturation: denaturation of the template inot single strands.
(ii) Annealing: annealing of primers to each original strand for new strand synthesis.
(iii) Extension:extension of new DNA strands from the primers.


110.

Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends.

Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.

  • If both assertion and reason are true and reason is the correct explanation of assertion.

  • If both assertion and reason are true but reason is not the correct explanation of assertion.

  • If assertion is true but reason is false.

  • If both assertion and reason are false.


C.

If assertion is true but reason is false.

Restriction enzyme, a type of endonuclease, functions by "inspecting" the length of a DNA sequence. Once it finds a recognition sequence, it binds and cuts each of the two strands of the double helix at specific point leaving single stranded portions at the ends. This results in overhanging stretches called sticky ends. These are named so because they form hydrogen bonds with their complementary counter parts, i.e. they can join similar complementary end of DNA fragment from some other source with the help of DNA ligase. This stickiness of the ends facilitates the action of the enzyme DNA ligase, not DNA polymerase.