Describe internal factors affecting photosynthesis.

Internal factors affecting photosynthesis are :

1. The number, size , age and orientation of the leaves, mesophyll cells and chloroplasts

2. Internal C02 concentraion.

3. The amount of chlorophyll

4. Genetics and the growth of the plants. 

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Describe the effect of oxygen on photosynthesis.


Effect of oxygen on photosynthesis : Oxygen is not the raw material for photosynthesis, but in the absence of oxygen, photosynthesis cannot occur. A slight increase in the amount of oxygen reduces the rate of photosynthesis in C3 plants. Higher concentration of oxygen reduces the rate of photosynthesis in C3 and C4 plants. It is called Warburg’s effett.
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Prove that the oxygen comes from water and not from the carbon dioxide.

Van Niel’s observation : Van Niel observed that photosynthesis is a light-dependent reaction and the O2 comes from the water and not carbon dioxide.. In green plants H2O is the hydrogen donor and O2 is the product. whereas in purple and green bacteria the hydrogen donor is H2S and the product is sulphur.  


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Describe Robin Hill experiment.

Robin Hill experiment: He took the isolated chloroplasts of Stellaria and placed them in water having hydrogen acceptors (like chromates, ferricyanides, cytochromes, benzoquinones etc.) He illuminated water and the oxygen was liberated in the absence of carbondioxide. Describe Robin Hill experiment.

The hydrogen acceptors are called Hill oxidants and the process of liberation of oxygen in the absence of carbon dioxide is called Hill oxidation. In plants hydrogen acceptor is NADP+

Describe Robin Hill experiment.
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Write a note on photosynthetic unit.

The photosynthetic unit is a complex of pigments and proteins coupled to a reaction center where the initial light-driven charge separation of photosynthetic reactions takes place.
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