Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Action of restriction enzyme
EcoRI cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA.
Steps in formation of recombinant DNA by action of restriction endonuclease enzyme - EcoRI
(i) They provide optimum growth conditions (temperature, pH, substrate, salts, vitamins, oxygen) to achieve the desired product.
(ii) They are cost effective .
(iii) Due to baffles, oxygen transfer rate is very high
(iv) Capacity of stirred tank is more as compared to shake flasks.