What are BACs and YACs ?

BACs and YACs are artificial chromosomes from bacteria and yeast used for efficient gene transfer and gene sequencing.
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What is crown gall tumor ?

Crown Gall tumor is the disease of several Dicot plants caused by Agrobacterium tumefaciencs .It is characterised by cancerous overgrowths on roots and crown of plants and is thus called crown gall tumor
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What is the role of restriction endonuclease ?

Restriction endonucleases are specific enzymes which can cleave double stranded DNA at specific sites called recognition sequences. Restriction endonucleases provide protection to the bacteria by restricting the growth of bacteriophages. They also serve as tools for Recombinant DNA technology.

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Define recombinant DNA.


Recombinant DNA is the artificially created DNA which incorporates DNA segments from different sources into one.
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Define T-DNA.

T-DNA or the Transfer-DNA is the part of Ti plasmid that is transferred to the plant and  transforms the normal cells into tumour cells .
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