There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?

  • Coelom

  • Coenzyme

  • coli

  • coli


C.

coli

Restriction endonuclease recognizes a particular palindromic sequence and degrades the same. It was so, called because it restricted the growth of bacteriophase in the bacterium (e.g. E. coli). The convention for naming these enzymes is the first letter of the name comes from the bacterial genus; the second two letters come from the species, and the fourth letter from strain, e.g., numbers following the names indicate the order in which the enzymes were isoalted.

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Agarose extracted from sea weeds finds use in

  • tissue culture

  • PCR

  • gel electrophoresis

  • gel electrophoresis


C.

gel electrophoresis

Gel electrophoresis is a technique to separate fragments of DNA. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. Now-a-days the most commonly used matrix is agarose which is a natural polymer extracted from seaweeds (e.g., Gelidium, Gracilaria, Gigartina, etc.).

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Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it?
5' ______ GAATTC ________ 3'
3'________CTTAAG ________ 5'

  • Deletion mutation

  • Start codon at the 5'end

  • Palindromic sequence of base pairs

  • Palindromic sequence of base pairs


C.

Palindromic sequence of base pairs

Palindromic DNA is a base sequence of DNA, which reads the same forward or backward. It has similar sequence in both the strands. Different types of palindromic sequences are recognised by restriction endonucleases.
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Maximum number of existing transgenic animals is of 

  • mice 

  • cow

  • pig

  • pig


A.

mice 

animals that have had their DNA manipulated to possess and express an extra (foreign) gene are known as transgenic animals. e.g, rats,rabbits, pig, sheep, cows, fish etc. Over 95% of all eisting transgenic animals are mice.

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Which one of the following structural formulae of two organic compounds is correctly identified along with its related function?

  • A: Triglycerid e- major - source of energy

  • B: Uracil - a component of DNA

  • A - Lecithin - a component of cell membrane

  • A - Lecithin - a component of cell membrane


C.

A - Lecithin - a component of cell membrane

Lecithin is a phospholipid composed of choline and inositol. It is found in all living cells as a major component of cell membrane.

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