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The DNA fragments separated on an agarose gel can be visualised after staining with

  • Bromophenol blue

  • Acetocarmine

  • Aniline blue

  • Aniline blue


D.

Aniline blue

Ethidium bromide is used to stain the DNA fragments and will appear as orange coloured bands under UV light.

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In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is

  • Indian Council of Medical Research (ICMR)

  • Council for Scientific and Industrial Research (CSIR)

  • Genetic Engineering Appraisal Committee (GEAC)

  • Research Committee on Genetic Manipulation (RCGM)


C.

Genetic Engineering Appraisal Committee (GEAC)

Indian Government has setup organisation such as GEAC (Genetic Engineering Appraisal Committee) which will make decisions regarding the validity of GM research and safety of introducing GM-organism for public services.


The correct order of steps in Polymerase Chain Reaction (PCR) is

  • Extension, Denaturation, Annealing

  • Annealing, Extension, Denaturation

  • Denaturation, Annealing, Extension

  • Denaturation, Extension, Annealing


C.

Denaturation, Annealing, Extension

PCR is based on three steps:
(i) Denaturation: denaturation of the template inot single strands.
(ii) Annealing: annealing of primers to each original strand for new strand synthesis.
(iii) Extension:extension of new DNA strands from the primers.


Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called

  • Bio-infringement

  • Biopiracy

  • Bioexploitation

  • Biodegradation


B.

Biopiracy

Biopiracy is a term used for or refers to the use of bioresources by multinational companies and other organisation without proper authorisation from the countries and people concerned with compensatory payment.


Which of these statements is incorrect?

  • Enzymes of TCA cycle are present in mitochondrial matrix

  • Glycolysis occurs in cytosol

  • Oxidative phosphorylation takes place in outer mitochondrial membrane

  • Glycolysis operates as long as it is supplied with NAD that can pick up hydrogen atoms


C.

Oxidative phosphorylation takes place in outer mitochondrial membrane

Oxidative phosphorylation takes place in inner mitochondrial membrane.


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