Farmers in a particular region were concerned that pre-mature yellowing of leaves of a pulse crop might cause decrease in the yield. Which treatment could be most beneficial to obtain maximum seed yield?

  • Frequent irrigation of the crop

  • Treatment of the plants with cytokinins alongwith a small dose of nitrogenous fertilizer

  • Removal of all yellow leaves and spraying the remaining green leaves with 2,4,5- trichlorophenoxy acetic acid

  • Removal of all yellow leaves and spraying the remaining green leaves with 2,4,5- trichlorophenoxy acetic acid


D.

Removal of all yellow leaves and spraying the remaining green leaves with 2,4,5- trichlorophenoxy acetic acid

If a pulse crop possesses premature yellowing of leaves and decrease in yield and application of magnesium and iron to promote synthesis of chlorophyll may become most beneficial to overcome the problem and to obtain maximum seed yield.
Magnesium is an important part of ring structure of chlorophyll molecule and its deficiency causes chlorosis and premature leaf abscission.
In iron deficiency also, the leaves become chlorotic because iron is required for the synthesis of some of the chlorophyll protein complexes in the chloroplast.

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Read the following four statements (I - IV) about certain mistakes in two of them
I .The first transgenic buffalo, Rosie produced mile which was human alpha - lactal bumin enriched
II. Restriction enzymes are used in isolation of DNA from another macro -molecules.
III. downstream processing is one of the steps of R-DNA technology.
IV. Disarmed pathogen vectors are also used in the transfer of R - DNA into the host. 
Which are the two statements having mistakes?

  • Statement (II) and (III)

  • Statements (III) and (IV)

  • Statements (I) and (III)

  • Statements (I) and (III)


D.

Statements (I) and (III)

Transgenic Rosiae is actually Cow. It is first transgenic cow produced in 1997.

Restriction enzymes cut the DNA at a specific site. The separation of DNA is performed by gel electrophoresis

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Microbes found to be very useful in genetic engineering are

  • Escherichia coli and Agrobacterium tumefaciens

  • Vibrio cholerae and a tailed bacteriophage

  • Diplococcus sp. and Pseudomonas sp

  • Diplococcus sp. and Pseudomonas sp


A.

Escherichia coli and Agrobacterium tumefaciens

Escherichia coli and Agrobacterium tumefaciens are the microbes found to be very useful in genetic engineering. 
E. coli is a motile, gram negative, rod shaped bacterium which is a normal inhabitant of human colon. It is most extensively used in bacterial genetics and molecular biology.
Agrobacterium tumefaciens is a soil bacterium. It has Ti plasmid (Tumour inducing plasmid) and it can be used for the transfer of a desired gene in dicot plants.

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Which one of the following proved effective for biological control of nematodal diseases in plants?

  • Pisolithus tinctorius

  • Pseudomonas cepacin

  • Gliocladium virens

  • Gliocladium virens


D.

Gliocladium virens

Paexcilomyces Lilacinus is proved effective for biological control of nematodal  disease in plants. it is easily produced in vitro, Rhizosphere competent, attack the eggs of several nematodes species and treatment of plant matter, eg, seed tuber, can be effective.

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Some of the characteristics of Bt cotton are 

  • long fibre and resistance of aphids

  • medium yield, long fibre and resistance to beetle pests

  • high yield and production of toxic protein crystals which kill dipteran pests

  • high yield and production of toxic protein crystals which kill dipteran pests


D.

high yield and production of toxic protein crystals which kill dipteran pests

Bacillus thuringiensis forms crystals containing a toxic insecticidal protein. Bt toxin protein exists as an inactive toxin, it is converted into an active form of toxin due to the alkaline pH of the gut, which solubilises the crystals. The activated toxin binds to the surface of midgut epithelial cells and creates pores the cause cell swelling and lysis and eventually cause the death of the insect. Specific Bt toxin genes were isolated from B. thruingiensis and incorporated into the several plants such as cotton. The toxin is coded by the gene name cry. There is a number of them, eg, the proteins encoded by the genes cry IAc a cry II Ab control the cotton bollworms, that of cry IAB control corn borers.

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