DNA and RNA segment tagged wit  a radioactive molecule is called

  • vector

  • probe

  • clone

  • clone


B.

probe

The probe is a defined nucleic acid molecule that can be used in molecular hybridization sequence that is complementary to it, by virtue of a label carried by the probe. The label may be radioactive or non- radioactive. Nucleic acid probes have a wide range of applications such as in the detection of microorganisms in clinical specimens, in food and water samples, in the detection of genetic diseases, etc.

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The one aspect which is not a salient feature of genetic code is its being

  • degenerate

  • ambiguous

  • universal

  • universal


D.

universal

The genetic code is degenerate, i.e, a given amino acid can be specified by more than one codons.
The genetic code inside the cell medium is said to be non-ambiguous because a particular condon always codes are same amino acid. But in certain rare cases the genetic code is found to be ambiguous, i.e some condon code for different amino acids under different conditions. The genetic code is almost universal.

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Which one of the following palindromic base sequences in DNA can be easily cut at about the middle be some particular restriction enzyme?

  • 5'-CGTTCG -3'
    3'-ATCCTA-5'

  • 5'-GATATG-3'
    3'-CTACTA-5'

  • 5'-GAATTC-3'
    3'CTTAAG-5'

  • 5'-GAATTC-3'
    3'CTTAAG-5'


C.

5'-GAATTC-3'
3'CTTAAG-5'

The palindromic base sequence is a sequence in double -stranded nucleic acids that read the same on both strands when reading one strand from left to right and the other from right to left (i.e both strands read 5' ->3'). Out of the given options

5' - GAATTC - 3'
3' - CTTAAG-5'

Is a palindromic sequence that can be cut at about the middle by a particular restriction enzyme.

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Which one of the following techniques made it possible to genetically engineer living organism

  • Recombinant DNA techniques

  • X -ray diffraction

  • Heavier isotope labelling

  • Heavier isotope labelling


A.

Recombinant DNA techniques

Recombinant DNA technology is joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations.

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Which one of the following is used as a vector for cloning the gene into higher organisms?

  • Baculovirus

  • Salmonella typhimurium

  • Rhizopus nigricans

  • Rhizopus nigricans


D.

Rhizopus nigricans

Retroviruses are RNA-containing animal viruses that replicate through a DNA intermediate.  Retroviruses in animals have the ability to transform normal cells into cancerous cells. A better understanding of the act of delivering genes by pathogen into useful vectors for delivering genes of interest of humans. Retroviruses have been disarmed and are now used to deliver desirable genes into animals cells.

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