Gel electrophoresis is used for  from Biology Molecular Basis of Inheritance Class 12 Nagaland Board
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Molecular Basis of Inheritance

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Biology

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Gel electrophoresis is used for 

  • cutting of DNA into fragments

  • separation of DNA fragments according to their size

  • construction of recombinant DNA by joining with cloning vectors

  • isolation of DNA molecule


B.

separation of DNA fragments according to their size

When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different size can be separated through gel electrophoresis. Gel electrophoresis involves the movement of fragments or molecules from a well created on edge of the gel.




When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different size can be separated through gel electrophoresis. Gel electrophoresis involves the movement of fragments or molecules from a well created on edge of the gel.




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Comment two chains of DNA have antiparallel polarity.

The Two chain of DNA are antiparallel that is
if one chain has 5’ x 3’ polarity the other chain has 3’ x 5’ polarity.
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List the function of RNA.

Functions of RNA
RNA acts as genetic material in viruses
It also functions as an adapter and messenger 
It also acts as a catalytic molecule and catalyses various biochemical reactions.
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How many nucleotides are present in a bacteriophage ϕ x 174.

5386
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Name the genetic material for majority of organisms.

DNA (Deoxyribose nucleic acid)
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List the number of base pairs in :

(i) lambda bacteriophage

(ii) E.coli and

(iii) haploid content of human DNA.


(i) 48502 bp
(ii) 4.6 x 106 bp and
(iii) 3.3 x 109 bp.
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