ï»¿ The linking of antibiotic resistance gene with the plasmid vector became possible with from Biology Molecular Basis of Inheritance Class 12 Nagaland Board

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The linking of antibiotic resistance gene with the plasmid vector became possible with

• DNA ligase

• endonucleases

• DNA polymerase

• exonucleases

A.

DNA ligase

DNA ligase is used to seal the nicks that remain in recombinant DNA molecule. In fact, DNA ligase joins together the neighbouring nucleotides flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecules is a vector into which the desired DNA fragment has been inserted to enable its cloning in an appropriate host.Â
Endonucleases are the enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which has specific base sequences, such endonucleases are known as restriction endonucleases.Â
Exonucleases are enzymes that remove one or more nucleotide from the free ends.

DNA polymerase is the enzyme, which causes polymerization of nucleotides during DNA replication.

DNA ligase is used to seal the nicks that remain in recombinant DNA molecule. In fact, DNA ligase joins together the neighbouring nucleotides flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecules is a vector into which the desired DNA fragment has been inserted to enable its cloning in an appropriate host.Â
Endonucleases are the enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which has specific base sequences, such endonucleases are known as restriction endonucleases.Â
Exonucleases are enzymes that remove one or more nucleotide from the free ends.

DNA polymerase is the enzyme, which causes polymerization of nucleotides during DNA replication.

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Name the genetic material for majority of organisms.

DNA (Deoxyribose nucleic acid)
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How many nucleotides are present in a bacteriophage $\mathrm{\varphi }$ x 174.

5386
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List the number of base pairs in :

(i) lambda bacteriophage

(ii) E.coli and

(iii) haploid content of human DNA.

(i) 48502 bp
(ii) 4.6 x 106 bp and
(iii) 3.3 x 109 bp.
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Comment two chains of DNA have antiparallel polarity.

The Two chain of DNA are antiparallel that is
if one chain has 5’ x 3’ polarity the other chain has 3’ x 5’ polarity.
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List the function of RNA.

Functions of RNA
RNA acts as genetic material in viruses
It also functions as an adapter and messenger
It also acts as a catalytic molecule and catalyses various biochemical reactions.
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