Kohler and Milstein developed biotechnology for :
monoclonal antibodies
steroid synthesis
immobilization of enzymes
myeloma
A.
monoclonal antibodies
Monoclonal antibodies are monospecific antibodies that are made by identical immune cells that are all clones of a unique parent cell.
In 1975, Georges Kohler and Cesar Milstein succeeded in making fusions of myeloma cell lines with B cells to produce hybridomas that made antibodies to known antigens. These are pure, high affinity antibodies, specific for an antigen which are obtained outside the body from clonal cultures of hybridoma cells.
The polymerase chain reaction1 (PCR) is a technique that is used for
in vivo replication of specific DNA sequence using thermostable DNA polymerase
in vitro synthesis of mRNA
in vitro replication of specific DNA sequence using thermostable DNA polymerase
in vivo synthesis of mRNA
in vivo synthesis of mRNA
In lysogenic phase which of the following takes place?
Phage DNA integrated with host genome and does not multiplied
Phage DNA does not integrate with host genome
Phage DNA integrates with host genome and bacterial cell does not lyse
Phage DNA integrated with host DNA and multiplied
Hybridoma technology is used to :
kill the cancer cells
formation of somaclonal antibodies
formation of somatic hybrids
formation of antibiotics
e -DNA is formed from m-RNA by which enzyme:
restriction endonuclease
reverse transcriptase
DNA polymerase
RNA polymerase
The first recombinant DNA was constructed by linking an antibiotic resistant gene with the native plasmid of V
Escherichia coli
Salmonella typhimurium
Clostridium butylicum
Acetobacter aceti
Acetobacter aceti
Bioreactors are useful in
Separation and purification of a product
Processing of large volumes of culture
Micro-injection
Isolation of genetic material
Isolation of genetic material
Plasmid is
small extrachromosomal circular self replicating DNA that can carry genes into host organism
bacteriophage
DNA found in mitochondria
DNA incorporated in bacterial chromosome
Introduction of foreign genes for improving genotype is
biotechnology
vernalization
tissue culture
genetic engineering
Science of engineering and technology applied to life sciences is
Biotechnology
Genetic engineering
Pathology
Biological science
