Multiple Choice Questions

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Introduction of foreign genes for improving genotype is

  • biotechnology

  • vernalization

  • tissue culture

  • genetic engineering


D.

genetic engineering

Genetic Engineering is the introduction of foreign genes for improving genotype. It is a technique of manipulating prokaryotic and eukaryotic DNA. It allows breaking of a DNA molecule at two desired places to isolate a specific DNA segment and then its insertion in another DNA molecule at a desired position. The product thus obtained is called r DNA and the technique as genetic engineering.


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Bioreactors are useful in

  • Separation and purification of a product

  • Processing of large volumes of culture

  • Micro-injection

  • Isolation of genetic material

  • Isolation of genetic material


In lysogenic phase which of the following takes place?

  • Phage DNA integrated with host genome and does not multiplied

  • Phage DNA does not integrate with host genome

  • Phage DNA integrates with host genome and bacterial cell does not lyse

  • Phage DNA integrated with host DNA and multiplied


Plasmid is

  • small extrachromosomal circular self replicating DNA that can carry genes into host organism

  • bacteriophage

  • DNA found in mitochondria

  • DNA incorporated in bacterial chromosome


Science of engineering and technology applied to life sciences is

  • Biotechnology

  • Genetic engineering

  • Pathology

  • Biological science


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e -DNA is formed from m-RNA by which enzyme:

  • restriction endonuclease

  • reverse transcriptase

  • DNA polymerase

  • RNA polymerase


Hybridoma technology is used to :

  • kill the cancer cells

  • formation of somaclonal antibodies

  • formation of somatic hybrids

  • formation of antibiotics


Kohler and Milstein developed biotechnology for :

  • monoclonal antibodies

  • steroid synthesis

  • immobilization of enzymes

  • myeloma


The first recombinant DNA was constructed by linking an antibiotic resistant gene with the native plasmid of V

  • Escherichia coli

  • Salmonella typhimurium

  • Clostridium butylicum

  • Acetobacter aceti

  • Acetobacter aceti


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The polymerase chain reaction1 (PCR) is a technique that is used for

  • in vivo replication of specific DNA sequence using thermostable DNA polymerase

  • in vitro synthesis of mRNA

  • in vitro replication of specific DNA sequence using thermostable DNA polymerase

  • in vivo synthesis of mRNA

  • in vivo synthesis of mRNA


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