Multiple Choice Questions

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Which one is a true statement regarding DNA polymerase used in PCR?
  • It is used to ligate introduced DNA in recipient cells

  • It serves as a selectable marker

  • It is isolated from a virus

  • It is isolated from a virus


D.

It is isolated from a virus

Polymerase Chain Reaction (PCR) is used to amplify a DNA segment or to synthesize in vitro the multiple copies of gene (or DNA) of interest, using two sets of primers and the enzyme DNA polymerase. This enzyme is isolated from a bacterium Thermus aquatics and it remains active during the high temperature but high temperature induced denaturation of double stranded DNA

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An analysis of chromosomal DNA using the Southern hybridization technique does not use

  • electrophoresis

  • blotting

  • autoradiography

  • autoradiography

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A single strand of nucleic acid tagged with a radioactive molecule is called

  • vector

  • selectable marker

  • plasmid

  • plasmid

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Removal of RNA polymerase -III from nucleoplasm will affect the synthesis of

  • tRNA

  • rRNA

  • mRNA

  • mRNA

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Removal of introns and joining of exons in a defined order during transcription in called

  • looping

  • inducing

  • slicing

  • slicing

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Which one of the following is not a part of a transcription unit in DNA?

  • The inducer

  • A terminator

  • A promoter

  • A promoter

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Ribosomal RNA is actively synthesised in 

  • lysosomes

  • nucleolus

  • nucleoplasm

  • nucleoplasm

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The first human hormone produced by recombinant DNA technology is 

  • insulin

  • estrogen

  • thyroxin

  • thyroxin

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Given below is the diagrammatic representation of one of the categories of small molecular weight organic compounds in the living tissues. Identify the category shown and the one blank component X in it

  • Category                       Component
    Nucleoside                         Uracil

  • Category                       Component
    Cholesterol                       Guanin
  • Category                       Component
    Amino acid                       NH2
  • Category                       Component
    Amino acid                       NH2
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PCR and Restriction Fragment Length Polymorphism are the methods for

  • Study of enzymes

  • Genetic transformation

  • DNA sequencing

  • DNA sequencing

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