Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
To obtain a foreign-gene product, following steps should be undertaken:
(i) Identification of DNA with desirable genes.
(ii) Introduction of the identified DNA into suitable host to form recombinant DNA (rDNA).
(iii) Maintenance of introduced DNA in particular host and gene cloning.
(iv) Transfer of the DNA (gene transfer) to its progeny.
(v) Selection of the recombinants from non-recombinants.
(vi) Expression of gene of interest by culturing recombinant cells.
(vii) Culturing of cells in bioreactors for large scale production of desired gene product.
During a medical investigation, an infant was found to possess an extra chromosome 21. Decribe the symptoms the child is likely to develop later in the life.
(a) List the three states the annuals and biennial angiosperms have to pass through during their life cycle.
(b) List and describe any two vegetative propagules in flowering plants.
A number of passengers were severely burnt beyond recognition during a train accident. Name and describe a modern technique that can help hand over the dead to their relatives.
(a) What precaution(s) would you recommend to a patient requiring repeated blood transfusion?
(b) If the advise is not followed by the patient there is an apprehension that the patient might contract a disease that would destroy the immune system of his/her body. Explain with the help of schematic diagram only how the immune system would get affected and destroyed.
(a) What is inbreeding depression?
(b) Explain the importance of ‘selection’ during inbreeding in cattle.
Explain the role(s) of the following in biotechnology.
(a) Restriction endonuclease
(b) Gel-electrophoresis
(c) Selectable markers in pBR322
