Gel electrophoresis is used for
Cutting of DNA into fragments
Separation of DNA fragments according to their size
Construction of recombinant DNA by joining with cloning vectors
Isolation of DNA molecule
B.
Separation of DNA fragments according to their size
When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves the movement of fragments or molecules on the basis if molecule size and shape under the influence of electric field.
Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. It cleaves DNA only within or near recognition sites, which have specific base sequences, such endonucleases are known as a restriction endonucleases.
The selected vector is cut open with a restriction enzyme that has a unique target site located in the sequence, where the DNA insert should be integrated to facilitate the selection of recombinant DNA.
In gymnosperms, the Ovule is naked because
Ovary wall is absent
Integuments are absent
Perianth is absen
Nucellus is absent
The rupture and fractionation do not usually occur in the water column in vessel/tracheids during the ascent of sap because of
Lignified thick walls
Cohesion and adhesion
Weak gravitational pull
Transpiration pull
Polysome is formed by
Several ribosomes attached to a single mRNA
Many ribosomes attached to a strand of endoplasmic reticulum
A ribosome with several sub-units
Ribosomes attached to each other in a linear arrangement
Core zone, buffer zone and manipulation zone. are found in
National park
Sanctuary
Tiger reserve
Biosphere reserve
Largest tiger population is found in
Sunderban national park
Corbett national park
Ranthambhore national park
Kanha national park
