Extraction with solvent (differential extraction): The method is based on the fact that organic substances are more soluble in organic solvents than in water. The organic substance is extracted from its aqueous solution adopting the following procedure:

1. The aqueous solution containing organic substance is shaken with a suitable organic solvent which dissolves the substance but is immiscible with water. Two layers are formed—organic layer and an aqueous layer.

2. The solvent layer containing the organic substance (organic layer) is separated using a separating funnel. The impurities remain in the aqueous layer.

3. The organic solvent is removed by distillation to obtain the organic substance.