The linking of antibiotic resistance gene with the plasmid vector became possible with
DNA ligase
endonucleases
DNA polymerase
DNA polymerase
A.
DNA ligase
DNA ligase is used to seal the nicks that remain in recombinant DNA molecule. In fact, DNA ligase joins together the neighbouring nucleotides flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecules is a vector into which the desired DNA fragment has been inserted to enable its cloning in an appropriate host.
Endonucleases are the enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which has specific base sequences, such endonucleases are known as restriction endonucleases.
Exonucleases are enzymes that remove one or more nucleotide from the free ends.
DNA polymerase is the enzyme, which causes polymerization of nucleotides during DNA replication.
Telomere repetitive DNA sequences control the function of eukaryotic chromosomes because they
act as replicons.
are RNA transcription initiator
help chromosome pairing
help chromosome pairing
Molecular basis of organ differentiation dpends on the modulation in transcription by
RNA polymerase
ribosome
transcription factor
transcription factor
During transcription, RNA polymerase holoenzyme binds to a gene promoter and assumes a saddle-like structure, what is it’s DNA-binding sequence?
TTAA
AATT
CACC
CACC
Differentiation of organs and tissues in a developing organism, is associated with
developmental mutations
differential expression of genes
lethal mutations
lethal mutations
