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In order to obtain virus-free plants through tissue culture the best method is

  • protoplast culture

  • embryo rescue

  • anther culture

  • anther culture


D.

anther culture

In meristem culture, the shoot apical meristem alongwith some surrounding tissue is grown in vitro. It is used for clonal propagation and recovery of virus free plants. It is also potentially useful in germ plasma exchange and long term storage of germ plasma through freeze preservation.

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Homozygous purelines in cattle can be obtained by

  • mating of related individuals of same breed

  • mating of unrelated individuals of same breed

  • mating of individuals of different breed

  • mating of individuals of different breed


In maize, hybrid vigour is exploited by

  • bombarding the protoplast with DNA

  • crossing of two inbreed parental lines

  • harvesting seeds from the most productive plants

  • harvesting seeds from the most productive plants


The process of separation and purification of expressed protein before marketing is called

  • Upstream processing

  • Downstream processing

  • Bioprocessing

  • Bioprocessing


Golden rice is a promising transgenic crop. When released for cultivation, it will help in

  • alleviation of vitamin A deficiency

  • pest resistance

  • herbicide tolerance

  • herbicide tolerance


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