A tumor inducing plasmid widely used in the production of transgenic plants is that of
Escherichia coli
Bacillus thuringiensis
Staphylococcus aureus
Agrobacterium tumefaciens
D.
Agrobacterium tumefaciens
Transgenic plants are plants that have been genetically engineered. It is a breeding approach that uses recombinant DNA techniques (RDT) to create plants with new characteristics.They are commonly identified as GMO or Genetically Modified Organisms. Agaraobacterium tumefaciens consists of Ti plasmid. It possess T- DNA which is responsible for causing tumour in host plant.
What is the source of EcoRI
Escherichia coli R I
Escherichia coli R I 13
Escherichia coli R Y 13
Escherichia coli RX 13
Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
If both assertion and reason are true and reason is the correct explanation of assertion
If both assertion and reason are true but reason is not the correct explanation of assertion
If assertion is true but reason is false
If both assertion and reason are false
How are transformants selected from nontransformants
Presence of more than one recognition site in the vector DNA.
Presence of alien DNA into the vector DNA results into insertional inactivation of selectable marker.
Antibiotic resistance gene gets inactivated due to insertion of alien DNA.
Both (b) and (c).
Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the breakdown of fungal cell, plant cell and bacterial cell respectively?
Lysozyme, lipases, trypsin
Chitinase, cellulase, lysozyme
Chitinase, cellulase, trypsin
Trypsin, lipases, cellulase
In cloning of cattle a fertilized egg is taken out of the mother's womb and
in the eight cell stage, cells are separated and cultured untill small embryos are formed which are implanted into the womb of other cows
in the eight cell stage the individual cells are separated under electrical field for further development in culture media
from this upto eight identical twins can be produced
the egg is divided into 4 pairs of cells which are implanted into the womb of other cows.
Which one of the following options is a correct match of phenomenon and its explanation
Reverse Transcription PCR - Many copies of a DNA sequence.
Central dogma - RNA > DNA > Protein > RNA.
RNA silencing - Use of ds-RNA to stop the expression of ss-RNA.
Transcription- Process of formation of RNA & proteins.
What is a plasmid?
Bacterial, linear, dsDNA
Extrachromosomal linear RNA
Extrachromosomal circular dsDNA
Autonomously replicating circular RNA
Which of the following is not a characteristic of pBR322 vector
It is the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez.
It is the most widely used, versatile and easily manipulated vector.
It has two antibiotic resistance genes, tetr and ampr.
It does not have restriction site for Sal I.
Which of the following is a cloning vector?
DNA of Salmonella typhimurium
Ti plasmid
Amp' and Tet' loci
Ori minus pBR322
