Specific radioactive identification of ribosomal RNA can be achieved by using C¹⁴ labelled

• Guanine

• Uracil

• thymine

• Cytocine

Which of the following statements is false regarding SDS-polyacrylamide gel electrophoresis?

• Proteins are separated by molecular weight

• SDS is a detergent which gives charge to protein

• Large protein move more slowly through gel

• SDS is used to maintain the 3-dimensional structure of protein

A bacterium divides after every 35 min, if a culture containing 10⁵ cells per mL is grown, then cell concentration per mL after 175 mins will be

• 175$\times$10⁵

• 125$\times$10⁵

• 48$\times$10⁵

• 32$\times$10⁵

Transformation in bacteria was discovered by

• Lederberg 

• Griffith

• Avery et. al

• Tatum

The arrangement of genes on chromosomes is

• linear 

• ovoid

• diffused 

• spiral

Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends.

Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.

• If both assertion and reason are true and reason is the correct explanation of assertion.

• If both assertion and reason are true but reason is not the correct explanation of assertion.

• If assertion is true but reason is false.

• If both assertion and reason are false.

Select the correct order of processing of PCR.

• Extension, primer annealing, denaturation

• Denaturation, primer annealing, extension

• Denaturation, extension, primer annealing

• Primer annealing, denaturation, extension

Plasmid vector in DNA recombinant technology means

• a virus that transfers gene to bacteria

• extra-chromosomal autonomously replicating circular DNA

• sticky end of DNA

• any fragment of DNA carrying desirable gene.

An example of gene therapy is

• production of injectable hepatitis B vaccine

• production of vaccines in food crops like potatoes which can be eaten

• introduction of gene for adenosine deaminase in persons suffering from Severe Combined Immuno Deficiency (SCID)

• production of test tube babies by artificial insemination and implantation of fertilised eggs

Assertion: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeasts (eukaryotes).

Reason: Both bacteria and yeasts multiply very fast to form huge populations which express the desired gene.

• If both assertion and reason are true and reason is the correct explanation of assertion. 

• If both assertion and reason are true but reason is not the correct explanation of assertion. 

• If assertion is true but reason is false. 

• If both assertion and reason are false.