Select the wrong statement
The presence of chromogenic substrate gives blue colour colonies, if the plasmid in the bacteria does not have an insert
Retroviruses in animals have the ability to transform
normal cells into cancerous cells
In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA
Since DNA is a hydrophilic molecule it cannot pass through cell membranes
Since DNA is a hydrophilic molecule it cannot pass through cell membranes
C.
In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA
Bacteria can be transformed in the following ways–
(i) By microinjections - where in DNA can be injected directly into the nucleus of cells to be transformed by using a micropipette. This technique was originally used in animal cells but has subsequently been applied to plant cells.
(ii) Gene gun/biolistic guns - Microscopic particles of gold or tungsten are coated with the DNA of interest & bombarded onto cells. This technique is known as biolistics.
Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. ELISA | 1. Antigen - antibody interaction |
| B. PCR | 2. Gene amplification |
| C. Biolistics | 3. Direct introduction of recombinant DNA |
| D. Micro- injection | 4. Gold coated DNA |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 4; B - 1; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. Bacillus thuringiensis | 1. Restriction endonuclease |
| B. Agrobacterium tumefaciens | 2. Thermostable DNA polymerase |
| C. Thermus aquaticus | 3. Insecticidal protein |
| D. Escherichia coli | 4. Tiplasmid |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 3; B - 4; C - 1; D - 2
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
The enzyme binds DNA at specific sites and cuts only one of the two strands.
The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
The enzyme cuts DNA molecule at identified position within the DNA.
