Multiple Choice QuestionsSelect the wrong statement
The presence of chromogenic substrate gives blue colour colonies, if the plasmid in the bacteria does not have an insert
Retroviruses in animals have the ability to transform
normal cells into cancerous cells
In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA
Since DNA is a hydrophilic molecule it cannot pass through cell membranes
Since DNA is a hydrophilic molecule it cannot pass through cell membranes
Microsatellites are
Repetitive DNA sequences
ESTs
YAC
BAC
BAC
A.
Repetitive DNA sequences
Every individual organism is unique. DNA sequences have some specific regions in DNA called repetitive DNA. These short nucleotide repeats vary in number from person to person and are called variable number of tandem repeat (VNTR). VNTR belongs to class of satellite DNA referred to as minisatellite. Size of VNTR varies from 1 to 20 bp.
Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. ELISA | 1. Antigen - antibody interaction |
| B. PCR | 2. Gene amplification |
| C. Biolistics | 3. Direct introduction of recombinant DNA |
| D. Micro- injection | 4. Gold coated DNA |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 4; B - 1; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. Bacillus thuringiensis | 1. Restriction endonuclease |
| B. Agrobacterium tumefaciens | 2. Thermostable DNA polymerase |
| C. Thermus aquaticus | 3. Insecticidal protein |
| D. Escherichia coli | 4. Tiplasmid |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 3; B - 4; C - 1; D - 2
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
The enzyme binds DNA at specific sites and cuts only one of the two strands.
The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
The enzyme cuts DNA molecule at identified position within the DNA.
Switch
