Match the items in Column I with their uses in Column II and choose the right option.

Column I	Column II
A. Bacillus thuringiensis	1. Restriction endonuclease
B. Agrobacterium tumefaciens	2. Thermostable DNA polymerase
C. Thermus aquaticus	3. Insecticidal protein
D. Escherichia coli	4. Tiplasmid

• A - 3; B - 4; C - 1; D - 2

• A - 2; B - 1; C - 4; D - 3

• A - 3; B - 4; C - 1; D - 2

• A - 1; B - 4; C - 2; D - 3

• A - 1; B - 4; C - 2; D - 3

Select the wrong statement

• The presence of chromogenic substrate gives blue colour colonies, if the plasmid in the bacteria does not have an insert

• Retroviruses in animals have the ability to transform
  normal cells into cancerous cells

• In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA

• Since DNA is a hydrophilic molecule it cannot pass through cell membranes

• Since DNA is a hydrophilic molecule it cannot pass through cell membranes

Microsatellites are

• Repetitive DNA sequences

• ESTs

• YAC

• BAC

• BAC

Match the items in Column I with their uses in Column II and choose the right option.

• A - 3; B - 4; C - 1; D - 2

• A - 2; B - 1; C - 4; D - 3

• A - 4; B - 1; C - 2; D - 3

• A - 1; B - 4; C - 2; D - 3

• A - 1; B - 4; C - 2; D - 3

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

• The enzyme binds DNA at specific sites and cuts only one of the two strands.

• The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

• The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.

• The enzyme cuts DNA molecule at identified position within the DNA.