Four techniques where the principle of ex-situ conservation of biodiversity has been employed are as follows:
i. Preservation of the gametes of threatened species (using cryopreservation techniques) in viable and fertile condition for long periods.
ii. In-vitrofertilisation of eggs.
iii. Tissue culture techniques used for the propagation of plants.
iv. Seed banks are used to keep the seeds of different genetic strains that are commercially important plants for long periods.
Rearrange the following in the current sequences to accomplish an important biotechnological reaction :
(a) In vitro synthesis of region of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
A heavily bleeding and bruised road accident victim was brought to a nursing home. The doctor immediately gave him an injection to protect him against a deadly disease.
(a) Write what did the doctor inject into the patient's body.
(b) How do you think this injection would protect the patient against the disease?
(c) Name the disease against which this injection was given and the kind of immunity it provides.
During a monohybrid cross involving a tall pea plant with a dwarf pea plant, the offspring populations were tall and dwarf in equal ratio. Work out a cross to show how is it possible.
