Short Answer Type

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Explain the significance of satellite DNA in DNA fingerprinting technique.


Satellite DNA consists of highly repetitive and generally non-coding DNA. It is very significant in DNA fingerprinting for the following reasons:

i. It shows high degree of polymorphism which forms the basis of DNA fingerprinting

ii. Different tissues in the body show the same degree of polymorphism and thus are useful as an identification tool in the forensic application.

iii. They are inheritable from parents to offsprings and  can be used for paternity testing.

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List any four techniques where the principle of ex-situ conservation of biodiversity has been employed.
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State what is apomixis. Comment on its significance. How can it be commercially used?

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During a monohybrid cross involving a tall pea plant with a dwarf pea plant, the offspring populations were tall and dwarf in equal ratio. Work out a cross to show how is it possible.

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What does the following equation represent? Explain
p2 + 2 pq + q2 = 1

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A heavily bleeding and bruised road accident victim was brought to a nursing home. The doctor immediately gave him an injection to protect him against a deadly disease.

(a) Write what did the doctor inject into the patient's body.
(b) How do you think this injection would protect the patient against the disease?
(c) Name the disease against which this injection was given and the kind of immunity it provides.

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Enumerate any six essentials of good, effective Dairy Farm Management Practices.

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State the medicinal value and the bioactive molecules produced by Streptococcus, Monascus and Trichoderma.
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What are methanogens ? How do they help to generate biogas?

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Rearrange the following in the current sequences to accomplish an important biotechnological reaction :

(a) In vitro synthesis of region of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

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