Explain with the help of a suitable example the naming of a restriction endonuclease.
The naming of a restriction endonuclease is done in the following way:
i) Ist letter represents the genus of the organism from which the enzyme is derived.
ii) IInd and IIIrd letters represent the species of the organism.
iii) IVth letter represents the name of the strain.
iv) Roman number represents order in which the enzyme was isolated.
For example, In EcoRI is Derived from genus Escherichia and species coli of, strain R.
It is the 1st to be discovered.
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
The enzyme binds DNA at specific sites and cuts only one of the two strands.
The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
The enzyme cuts DNA molecule at identified position within the DNA.
Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. ELISA | 1. Antigen - antibody interaction |
| B. PCR | 2. Gene amplification |
| C. Biolistics | 3. Direct introduction of recombinant DNA |
| D. Micro- injection | 4. Gold coated DNA |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 4; B - 1; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. Bacillus thuringiensis | 1. Restriction endonuclease |
| B. Agrobacterium tumefaciens | 2. Thermostable DNA polymerase |
| C. Thermus aquaticus | 3. Insecticidal protein |
| D. Escherichia coli | 4. Tiplasmid |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 3; B - 4; C - 1; D - 2
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
