Biotechnology : Principles and Processes

A tumor inducing plasmid widely used in the production of transgenic plants is that of

• Escherichia coli

• Bacillus thuringiensis

• Staphylococcus aureus

• Agrobacterium tumefaciens

Restriction endonuclease

• cuts the DNA molecule randomly

• cuts the DNA molecule at specific sites

• restricts the synthesis of DNA inside the nucleus

• restricts the synthesis of DNA inside the nucleus

Kohler and Milstein developed biotechnology for :

• monoclonal antibodies

• steroid synthesis

• immobilization of enzymes

• myeloma

What is the first step in the Southern Blot technique

• Denaturation of DNA on the gel for hybridization with specific probe

• Production of a group of genetically identical cells

• Digestion of DNA by restriction enzyme

• Isolation of DNA from a nucleated cell such as the one from the scene of crime

cDNA probes are copied from the messenger RNA molecules with the help of

• restriction enzymes

• reverse transcriptase

• DNA polymerase

• adenosine deaminase

Restriction enzymes:

• are endonucleases which cleave DNA at specific sites

• make DNA complementary to an existing DNA or RNA

• cut or join DNA fragments

• are required in vectorless direct gene transfer.

Introduction of foreign genes for improving genotype is

• biotechnology

• vernalization

• tissue culture

• genetic engineering

Hybridoma technology is used to :

• kill the cancer cells

• formation of somaclonal antibodies

• formation of somatic hybrids

• formation of antibiotics

Assertion : In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).

Reason : Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.

• If both Assertion and Reason are true and the Reason is the correct explanation of the Assertion

• If both Assertion and Reason are true but the Reason is not the correct explanation of the assertion

• If Assertion is true but Reason is false.

• If both Assertion and Reason are false

e -DNA is formed from m-RNA by which enzyme:

• restriction endonuclease

• reverse transcriptase

• DNA polymerase

• RNA polymerase