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 Multiple Choice QuestionsMultiple Choice Questions


A tumor inducing plasmid widely used in the production of transgenic plants is that of

  • Escherichia coli

  • Bacillus thuringiensis

  • Staphylococcus aureus

  • Agrobacterium tumefaciens


Restriction endonuclease

  • cuts the DNA molecule randomly

  • cuts the DNA molecule at specific sites

  • restricts the synthesis of DNA inside the nucleus

  • restricts the synthesis of DNA inside the nucleus



Kohler and Milstein developed biotechnology for :

  • monoclonal antibodies

  • steroid synthesis

  • immobilization of enzymes

  • myeloma


What is the first step in the Southern Blot technique

  • Denaturation of DNA on the gel for hybridization with specific probe

  • Production of a group of genetically identical cells

  • Digestion of DNA by restriction enzyme

  • Isolation of DNA from a nucleated cell such as the one from the scene of crime


cDNA probes are copied from the messenger RNA molecules with the help of

  • restriction enzymes

  • reverse transcriptase

  • DNA polymerase

  • adenosine deaminase


Restriction enzymes:

  • are endonucleases which cleave DNA at specific sites

  • make DNA complementary to an existing DNA or RNA

  • cut or join DNA fragments

  • are required in vectorless direct gene transfer.


Introduction of foreign genes for improving genotype is

  • biotechnology

  • vernalization

  • tissue culture

  • genetic engineering


Hybridoma technology is used to :

  • kill the cancer cells

  • formation of somaclonal antibodies

  • formation of somatic hybrids

  • formation of antibiotics


Assertion : In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).

Reason : Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.

  • If both Assertion and Reason are true and the Reason is the correct explanation of the Assertion

  • If both Assertion and Reason are true but the Reason is not the correct explanation of the assertion

  • If Assertion is true but Reason is false.

  • If both Assertion and Reason are false


e -DNA is formed from m-RNA by which enzyme:

  • restriction endonuclease

  • reverse transcriptase

  • DNA polymerase

  • RNA polymerase