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Describe briefly the following :

(a) Origin of replication (b) Bioreactor (c) Downstream processing


(a) Origin of replication (Ori).
1. It is a specific sequence of DNA bases, which  initiates replication.A prokaryotic DNA has normally a single oringin of replication, while eukaryotic DNA may have more than one. This sequence controls the copy number of linked DNA. An alien DNA for replication should be linked to the origin of replication

(b) Bioreactor are vessels in which raw materials are biologically converted into specific products using microbial, plant or human cells. It provides optimal conditions for achieving the desired product by providing optimum growth conditions, pH, substrate salts, vitamins, oxygen, etc.

(c) Downstream processing.are the series of processes which a genetically modified product undergoes before it is ready to be marketed. It generally involves processes like  separation and  purification.

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Can you list 10 recombinant proteins which are used in medical practice ? Find where they are used as therapeutics (use the internet).

Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.


Can you recall meiosis and indicate at what stage a recombinant DNA is made?


Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?


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