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The correct order of steps in Polymerase Chain Reaction (PCR) is

  • Extension, Denaturation, Annealing

  • Annealing, Extension, Denaturation

  • Denaturation, Annealing, Extension

  • Denaturation, Extension, Annealing


C.

Denaturation, Annealing, Extension

PCR is based on three steps:
(i) Denaturation: denaturation of the template inot single strands.
(ii) Annealing: annealing of primers to each original strand for new strand synthesis.
(iii) Extension:extension of new DNA strands from the primers.


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Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called

  • Bio-infringement

  • Biopiracy

  • Bioexploitation

  • Biodegradation


In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is

  • Indian Council of Medical Research (ICMR)

  • Council for Scientific and Industrial Research (CSIR)

  • Genetic Engineering Appraisal Committee (GEAC)

  • Research Committee on Genetic Manipulation (RCGM)


The DNA fragments separated on an agarose gel can be visualised after staining with

  • Bromophenol blue

  • Acetocarmine

  • Aniline blue

  • Aniline blue


Which of these statements is incorrect?

  • Enzymes of TCA cycle are present in mitochondrial matrix

  • Glycolysis occurs in cytosol

  • Oxidative phosphorylation takes place in outer mitochondrial membrane

  • Glycolysis operates as long as it is supplied with NAD that can pick up hydrogen atoms


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