Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. Bacillus thuringiensis | 1. Restriction endonuclease |
| B. Agrobacterium tumefaciens | 2. Thermostable DNA polymerase |
| C. Thermus aquaticus | 3. Insecticidal protein |
| D. Escherichia coli | 4. Tiplasmid |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 3; B - 4; C - 1; D - 2
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
E.
A - 3; B - 4; C - 2; D - 1
| Column I | Column II |
| A. Bacillus thuringiensis | 3. Restriction endonuclease |
| B. Agrobacterium tumefaciens | 4. Thermostable DNA polymerase |
| C. Thermus aquaticus | 2. Insecticidal protein |
| D. Escherichia coli | 1. Tiplasmid |
Explain with the help of a suitable example the naming of a restriction endonuclease.
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
The enzyme binds DNA at specific sites and cuts only one of the two strands.
The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
The enzyme cuts DNA molecule at identified position within the DNA.
Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. ELISA | 1. Antigen - antibody interaction |
| B. PCR | 2. Gene amplification |
| C. Biolistics | 3. Direct introduction of recombinant DNA |
| D. Micro- injection | 4. Gold coated DNA |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 4; B - 1; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
