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Microbes found to be very useful in genetic engineering are

  • Escherichia coli and Agrobacterium tumefaciens

  • Vibrio cholerae and a tailed bacteriophage

  • Diplococcus sp. and Pseudomonas sp

  • Diplococcus sp. and Pseudomonas sp


A.

Escherichia coli and Agrobacterium tumefaciens

Escherichia coli and Agrobacterium tumefaciens are the microbes found to be very useful in genetic engineering. 
E. coli is a motile, gram negative, rod shaped bacterium which is a normal inhabitant of human colon. It is most extensively used in bacterial genetics and molecular biology.
Agrobacterium tumefaciens is a soil bacterium. It has Ti plasmid (Tumour inducing plasmid) and it can be used for the transfer of a desired gene in dicot plants.

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Which one of the following proved effective for biological control of nematodal diseases in plants?

  • Pisolithus tinctorius

  • Pseudomonas cepacin

  • Gliocladium virens

  • Gliocladium virens


Farmers in a particular region were concerned that pre-mature yellowing of leaves of a pulse crop might cause decrease in the yield. Which treatment could be most beneficial to obtain maximum seed yield?

  • Frequent irrigation of the crop

  • Treatment of the plants with cytokinins alongwith a small dose of nitrogenous fertilizer

  • Removal of all yellow leaves and spraying the remaining green leaves with 2,4,5- trichlorophenoxy acetic acid

  • Removal of all yellow leaves and spraying the remaining green leaves with 2,4,5- trichlorophenoxy acetic acid


Some of the characteristics of Bt cotton are 

  • long fibre and resistance of aphids

  • medium yield, long fibre and resistance to beetle pests

  • high yield and production of toxic protein crystals which kill dipteran pests

  • high yield and production of toxic protein crystals which kill dipteran pests


Read the following four statements (I - IV) about certain mistakes in two of them
I .The first transgenic buffalo, Rosie produced mile which was human alpha - lactal bumin enriched
II. Restriction enzymes are used in isolation of DNA from another macro -molecules.
III. downstream processing is one of the steps of R-DNA technology.
IV. Disarmed pathogen vectors are also used in the transfer of R - DNA into the host. 
Which are the two statements having mistakes?

  • Statement (II) and (III)

  • Statements (III) and (IV)

  • Statements (I) and (III)

  • Statements (I) and (III)


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