State how Agrobacterium tumifaciens has been made a useful cloning vector to transfer DNA to plant cells.
Agrobacterium is a pathogen that affects several dicots and delivers a piece of DNA known as ‘T-DNA’ to transform normal cells to tumor cells. This potential of Agrobacterium has been used to transfer desired gene into the plant. The desired gene is inserted in the T-DNA region and the bacterium is allowed to infect the plant. In this way, the desired changes are made in the plant.
Which of the following is true for Golden rice?
It is pest resistant, with a gene from Bacillus thuringiensis
It is drought tolerant, developed using Agrobacterium vector
It has yellow grains, because of a gene introduced from a primitive variety of rice
It is Vitamin A enriched, with a gene from daffodil
How are 'sticky ends' formed on a DNA strand? Why are they so called?
What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in boll worm?
Moist surface of midgut
Alkaline pH of gut
Acidic pH of stomach
Body temperature
Mention the type of host cells suitable for the gene guns to introduce an alien DNA.
