How are 'sticky ends' formed on a DNA strand? Why are they so called?
Sticky ends are produced by restriction enzymes. These enzymes cut the strand of DNA a little away from the centre of the palindrome sites but between the same two bases on the opposite strands. This leaves single-stranded portions at the ends. These overhanging stretches are called 'sticky ends'.
These are called sticky ends because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.
Which of the following is true for Golden rice?
It is pest resistant, with a gene from Bacillus thuringiensis
It is drought tolerant, developed using Agrobacterium vector
It has yellow grains, because of a gene introduced from a primitive variety of rice
It is Vitamin A enriched, with a gene from daffodil
Mention the type of host cells suitable for the gene guns to introduce an alien DNA.
State how Agrobacterium tumifaciens has been made a useful cloning vector to transfer DNA to plant cells.
What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in boll worm?
Moist surface of midgut
Alkaline pH of gut
Acidic pH of stomach
Body temperature
