Which of the following is a restriction endonuclease?
Protease
DNase I
RNase
RNase
D.
RNase
Hind II is a restriction endonuclease are enzymes used for cutting DNA at specific locations.
(a) Describe the various steps of Griffith’s experiment that led to the conclusion of the ‘Transforming Principle’.
(b) How did the chemical nature of the ‘Transforming Principle’ get established?
If a desired gene is identified in an organism for some experiments, explain the process of the following:
(i) Cutting this desired gene at specific location
(ii) Synthesis of multiple copies of this desired gene
A. Describe the characteristics a cloning vector must possess.
B. Why DNA cannot pass through the cell membrane? Explain. How is a bacterial cell made competent to take up recombinant DNA from the medium?
Which of the following is not a feature of the plasmids?
Circular structure
Transferable
Single stranded
Single stranded
