140.
Find out a qualitative test for proteins, fats, oils, amino acids and test any fruit juice, saliva, sweat and urine for them.
The different tests are
(a) For proteins - Biuret's test.
If Biuret’s reagent is added to protein,and the colour of the reagent changes from light blue to purple it shows the presence of protein.(b) For fats or oils - Grease and solubility of the test.
(c) For amino acids - Ninhydrin test. I
f Ninhydrin reagent is added to the solution, then the colourless solution changes to pink, blue, or purple, it shoes the presence of amino acids.
On testing the fruit juice, saliva, sweat and urine for the molecules we get the following result.
| Substance |
Biuret's test for protein |
Grease or solubulity test for fats and oil |
Ninhydrin test for amino acids. |
| 1. Fruit juice |
Gives positive result showing the presence of proteins. |
Negative result showing absence of fats and oils. |
Gives positive result for the presence of amino acids. |
| 2. Saliva |
Shows presence of proteins |
Absence of fats and oils |
Shows presence of amino acids. |
| 3. Sweat |
Absence of proteins |
Presence of fats or opils |
Absence of amino acids |
| 4. Urine |
Presence of proteins. |
Not present |
Presence of amino-acids. |
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