Multiple Choice QuestionsThe cutting of DNA at specific locations become possible with the discovery of
Restriction enzymes
Probes
Selectable markers
Selectable markers
A.
Restriction enzymes
Restricition enzymes are DNA cutting enzymes found in bacteria. A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides. For example, the bacterium Haemophilus aegyptius produces an enzyme named Hae III that cuts DNA wherever, it encounters the sequence.
5'-G G C-3'
3'-C C G G-5'
The introduction of t-DNA into plates involves
Infection of the plant by agrobacterium tumefaciens
Altering the pH of the soil, then heat-shocking the plants
Exposing the plates to cold for a brief period
Exposing the plates to cold for a brief period
The DNA molecule to which the gene of interest is integrated for cloning is called
Transformer
Vector
Template
Template
The figure below is the diagrammatic representation o the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components(s)?
Ori- original restriction enzyme
rop-reduced osmotic pressure
Hind III, Eco RI-selectable markers
Hind III, Eco RI-selectable markers
Which one of the following is a case of wrong matching?
Somatic hybridization - Fusion of two diverse cells
Vector DNA - Site for tRNA synthesis
Micropropagation - In vitro production of plants in large numbers
Micropropagation - In vitro production of plants in large numbers
For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of
silver or platinum
platinum or zinc
silicon or platinum
silicon or platinum
Biolistics (gene-gun) is suitbale for
Disarming pathogen vectors
Transformation of plant cells
Constructing recombinant DNA by joing whith vectors
Constructing recombinant DNA by joing whith vectors
In genetic engineering, the antibiotics are used
as selectable markers
to select healthy vectors
as sequences from where replicaton starts
as sequences from where replicaton starts
Which one of the following represents a palindromic sequence in DNA?
5´ - GATACC - 3´
3´ - CCTAAG - 5
5´ - GAATTC - 3
3´ - CTTAAG - 5´
5´- CCAATG - 3´
3´ - GAATCC - 5´
5´- CCAATG - 3´
3´ - GAATCC - 5´
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