The correct order of steps in Polymerase Chain Reaction (PCR) is

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131.

A gene whose expression helps to identify transformed cell is known as

  • Selectable marker

  • Vector

  • Plasmid

  • Plasmid

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132.

The DNA fragments separated on an agarose gel can be visualised after staining with

  • Bromophenol blue

  • Acetocarmine

  • Aniline blue

  • Aniline blue

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133.

Which of these statements is incorrect?

  • Enzymes of TCA cycle are present in mitochondrial matrix

  • Glycolysis occurs in cytosol

  • Oxidative phosphorylation takes place in outer mitochondrial membrane

  • Glycolysis operates as long as it is supplied with NAD that can pick up hydrogen atoms


134.

Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called

  • Bio-infringement

  • Biopiracy

  • Bioexploitation

  • Biodegradation


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135.

In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is

  • Indian Council of Medical Research (ICMR)

  • Council for Scientific and Industrial Research (CSIR)

  • Genetic Engineering Appraisal Committee (GEAC)

  • Research Committee on Genetic Manipulation (RCGM)


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136.

The correct order of steps in Polymerase Chain Reaction (PCR) is

  • Extension, Denaturation, Annealing

  • Annealing, Extension, Denaturation

  • Denaturation, Annealing, Extension

  • Denaturation, Extension, Annealing


C.

Denaturation, Annealing, Extension

PCR is based on three steps:
(i) Denaturation: denaturation of the template inot single strands.
(ii) Annealing: annealing of primers to each original strand for new strand synthesis.
(iii) Extension:extension of new DNA strands from the primers.


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137.

All monerons

  • Contain DNA and RNA

  • Demonstrate a long circular strand of DNA, not formed enclosed in a nuclear membrane

  • Are bacteria

  • All of the above


138.

Skin colour is controlled b

  • Single gene

  • 3 pairs of genes

  • 2 pairs of genes

  • 2 pairs of genes with an intragene


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139.

Gyms revoluta is popularly known as

  • Sago palm

  • Royal palm

  • Date palm

  • Sea palm


140.

In DNA, when AGCT occurs, their association is as per which of the following pair

  • A-G, C-T

  • A-T, G-C

  • A-C, G-T, A-C, E-T

  • All of these


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