Previous Year Papers

Download Solved Question Papers Free for Offline Practice and view Solutions Online.

Test Series

Take Zigya Full and Sectional Test Series. Time it out for real assessment and get your results instantly.

Test Yourself

Practice and master your preparation for a specific topic or chapter. Check you scores at the end of the test.

Biotechnology : Principles and Processes

Multiple Choice Questions

141.

A segment of DNA has 120 adenine and 120 cytosine bases. The total number of nucleotides present in the segment is

142.
Gel electrophoresis is used for

Cutting of DNA into fragments

Separation of DNA fragments according to their size

Construction of recombinant DNA by joining with cloning vectors

Isolation of DNA molecule

Separation of DNA fragments according to their size

When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves the movement of fragments or molecules on the basis if molecule size and shape under the influence of electric field.

Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. It cleaves DNA only within or near recognition sites, which have specific base sequences, such endonucleases are known as a restriction endonucleases.

The selected vector is cut open with a restriction enzyme that has a unique target site located in the sequence, where the DNA insert should be integrated to facilitate the selection of recombinant DNA.