Which one of the following is a reorganization site for restriction enzymeBam HI?
5ʹ– GAATTC – 3ʹ 3ʹ – CTTAAG – 5ʹ
5ʹ – GGATCC – 3ʹ 5ʹ – CCATGG – 3ʹ
5ʹ – GGATCC -– 3ʹ 3ʹ – CCTAGG – 5ʹ
5ʹ– GAATTC – 3ʹ 5ʹ– GTTAAC – 3
Which one of the following pair is not correct?
Mangolian idiocy – 21st chromosome
Patau syndrome – 13th chromosome
Cri-Du-Chat – 11th chromosome
Edward syndrome – 18th chromosome
Which one of the following pair is not correct?
Single-stranded DNA
Single-stranded RNA
Double-stranded DNA
None of the above
Consider these sentences regarding to the structure and nature of DNA.
(I) DNA has tow pyrimidine bases which contain single ring structure.
(II) In DNA, the composition of bases should be
(III) Each base pair of DNA is 3.4Å apart from the base pair.
(IV) The nucleosome model DNA packaging was proposed by Kornberg and Thomas.
I and III
I, II and III
II, III and IV
I, III and IV
D.
I, III and IV
Erwin Chargaff (1950) made quantitative analysis of DNA and showed that the composition of DNA molecules varies in complex ways depending upon the source of DNA. He proposed Base Equivalence rule or Chargaff's rule, which states that for double-stranded DNA, the proportion of adenine always equals to that of thymine and proportion of guanine always equals to that of cytosine.
i.e. A=T and G=C.
It follows that, an equal proportion of purines and pyrimidines is always seen in a double-stranded DNA
i.e.
Specific radioactive identification of ribosomal RNA can be achieved by using C14 labelled
Guanine
Uracil
thymine
Cytocine
Which of the following statements is false regarding SDS-polyacrylamide gel electrophoresis?
Proteins are separated by molecular weight
SDS is a detergent which gives charge to protein
Large protein move more slowly through gel
SDS is used to maintain the 3-dimensional structure of protein
A bacterium divides after every 35 min, if a culture containing 105 cells per mL is grown, then cell concentration per mL after 175 mins will be
175105
125105
48105
32105
Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
If both assertion and reason are true and reason is the correct explanation of assertion.
If both assertion and reason are true but reason is not the correct explanation of assertion.
If assertion is true but reason is false.
If both assertion and reason are false.