Multiple Choice QuestionsSelect the correct order of processing of PCR.
Extension, primer annealing, denaturation
Denaturation, primer annealing, extension
Denaturation, extension, primer annealing
Primer annealing, denaturation, extension
Plasmid vector in DNA recombinant technology means
a virus that transfers gene to bacteria
extra-chromosomal autonomously replicating circular DNA
sticky end of DNA
any fragment of DNA carrying desirable gene.
An example of gene therapy is
production of injectable hepatitis B vaccine
production of vaccines in food crops like potatoes which can be eaten
introduction of gene for adenosine deaminase in persons suffering from Severe Combined Immuno Deficiency (SCID)
production of test tube babies by artificial insemination and implantation of fertilised eggs
Assertion: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeasts (eukaryotes).
Reason: Both bacteria and yeasts multiply very fast to form huge populations which express the desired gene.
If both assertion and reason are true and reason is the correct explanation of assertion.
If both assertion and reason are true but reason is not the correct explanation of assertion.
If assertion is true but reason is false.
If both assertion and reason are false.
A.
If both assertion and reason are true and reason is the correct explanation of assertion.
In recombinant DNA technology, widely used hosts for replication and amplification of recombinant DNA are prokaryotic E. coli and the eukaryotic yeast. They replicate very fast to form huge populations which express the desired gene. Yeast artificial chromosomes (YACs) are important cloning tools for the analysis of complex genome such as that of humans. They allow the maintenance, propagation and analysis of such genome in an experimentally tractable system, the yeast.
Which of the following is a cloning vector?
DNA of Salmonella typhimurium
Ti plasmid
Amp' and Tet' loci
Ori minus pBR322
In cloning of cattle a fertilized egg is taken out of the mother's womb and
in the eight cell stage, cells are separated and cultured untill small embryos are formed which are implanted into the womb of other cows
in the eight cell stage the individual cells are separated under electrical field for further development in culture media
from this upto eight identical twins can be produced
the egg is divided into 4 pairs of cells which are implanted into the womb of other cows.
How are transformants selected from nontransformants
Presence of more than one recognition site in the vector DNA.
Presence of alien DNA into the vector DNA results into insertional inactivation of selectable marker.
Antibiotic resistance gene gets inactivated due to insertion of alien DNA.
Both (b) and (c).
Which of the following is not a characteristic of pBR322 vector
It is the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez.
It is the most widely used, versatile and easily manipulated vector.
It has two antibiotic resistance genes, tetr and ampr.
It does not have restriction site for Sal I.
What is a plasmid?
Bacterial, linear, dsDNA
Extrachromosomal linear RNA
Extrachromosomal circular dsDNA
Autonomously replicating circular RNA
Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the breakdown of fungal cell, plant cell and bacterial cell respectively?
Lysozyme, lipases, trypsin
Chitinase, cellulase, lysozyme
Chitinase, cellulase, trypsin
Trypsin, lipases, cellulase
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