Multiple Choice QuestionsIntroduction of foreign genes for improving genotype is
biotechnology
vernalization
tissue culture
genetic engineering
The first recombinant DNA was constructed by linking an antibiotic resistant gene with the native plasmid of V
Escherichia coli
Salmonella typhimurium
Clostridium butylicum
Acetobacter aceti
Acetobacter aceti
The polymerase chain reaction1 (PCR) is a technique that is used for
in vivo replication of specific DNA sequence using thermostable DNA polymerase
in vitro synthesis of mRNA
in vitro replication of specific DNA sequence using thermostable DNA polymerase
in vivo synthesis of mRNA
in vivo synthesis of mRNA
Bioreactors are useful in
Separation and purification of a product
Processing of large volumes of culture
Micro-injection
Isolation of genetic material
Isolation of genetic material
Select the wrong statement
The presence of chromogenic substrate gives blue colour colonies, if the plasmid in the bacteria does not have an insert
Retroviruses in animals have the ability to transform
normal cells into cancerous cells
In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA
Since DNA is a hydrophilic molecule it cannot pass through cell membranes
Since DNA is a hydrophilic molecule it cannot pass through cell membranes
Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. ELISA | 1. Antigen - antibody interaction |
| B. PCR | 2. Gene amplification |
| C. Biolistics | 3. Direct introduction of recombinant DNA |
| D. Micro- injection | 4. Gold coated DNA |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 4; B - 1; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
Match the items in Column I with their uses in Column II and choose the right option.
| Column I | Column II |
| A. Bacillus thuringiensis | 1. Restriction endonuclease |
| B. Agrobacterium tumefaciens | 2. Thermostable DNA polymerase |
| C. Thermus aquaticus | 3. Insecticidal protein |
| D. Escherichia coli | 4. Tiplasmid |
A - 3; B - 4; C - 1; D - 2
A - 2; B - 1; C - 4; D - 3
A - 3; B - 4; C - 1; D - 2
A - 1; B - 4; C - 2; D - 3
A - 1; B - 4; C - 2; D - 3
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
The enzyme binds DNA at specific sites and cuts only one of the two strands.
The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
The enzyme cuts DNA molecule at identified position within the DNA.
A.
The enzyme binds DNA at specific sites and cuts only one of the two strands.
Restriction enzymes cut DNA molecules at a particular point by recognising a specific sequence. Each restriction endonuclease functions by inspecting the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar phosphate backbone.
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