Multiple Choice QuestionsCrystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because
Long Answer TypeDigrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
Short Answer TypeState how Agrobacterium tumifaciens has been made a useful cloning vector to transfer DNA to plant cells.
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Sticky ends are produced by restriction enzymes. These enzymes cut the strand of DNA a little away from the centre of the palindrome sites but between the same two bases on the opposite strands. This leaves single-stranded portions at the ends. These overhanging stretches are called 'sticky ends'.
These are called sticky ends because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.How did the process of RNA interference help to control the nematode from infecting the roots of tobacco plants?
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