Short Answer TypeWhy do lepidopterans die when they feed on Bt cotton plant? Explain how does it happen.
Explain the various steps involved in the production of artificial insulin.
* Insulin contains two short polypeptide chains: chain A and chain B linked together by disulphide bridges.
* In mammals insulin is synthesised as a pro-hormone. It contains an extra stretch called C-peptide.
* C-peptide is absent in the mature insulin and is removed during maturation into insulin.
* Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly in 1983. It prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli for production. The A and B chains produced were separated, extracted and combined, by creating disulfide bonds to form human insulin.
Multiple Choice QuestionsWhich of the following is not required for any of the techniques of DNA fingerprinting available at present?
Zinc finger analysis
Restriction enzymes
DNA-DNA hybridization
DNA-DNA hybridization
Which part of the tobacco plant is infected by Meloidogyne incognitia?
Leaf
Stem
Root
Root
Golden rice is a genetically modified crop plant where the incorporated gene i's meant for
Vitamin B
Vitamin C
Omega 3
Omega 3
A pleiotropic gene
is expressed only in primitive plants.
is a gene evolved during Pliocene
controls a trait only in combination with another gene
controls a trait only in combination with another gene
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