Biolistic technique is used in
tissue culture process
gene transfer process
hybridization process
germplasm conservation process
B.
gene transfer process
Electroporation and biolistics are two physical methods for gene transfection. In biolistics DNA is coated on to the surface of tiny metal particles usually tungeston or gold, which is then shot into the cells.
Electroporation is a molecular biology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs or DNA to be introduced into the cell.
Which one of the following bacterium is used for production of transgenic plants
Escherichia coli
Bacillus thuringiensis
Staphylococcus aureus
Agrobacterium tumefaciens
Basic principle of developing transgenic plants and animals is to introduce the gene of interest into the nucleus of
somatic cell
vegetative cell
germ cell
body cell
Which of the following Bt crops is being grown in India by the farmers?
Maize
Cotton
Brinjal
Soyabean
Insect pest resistant Bt cotton plant was developed using
somaclonal variation
micropropagation
somatic hybridisation
Transgenic technology
Genetically improved crop varieties can be developed in laboratory by
somatic hybridisation
transgenic technology
cell suspension culture
somaclonal variation
Which one of the following statements is wrong in relation to transgenic Bt cotton plant?
Crop yield loss due to the attack by Bacillus thuringiensis bacterium is reduced
Crop yield loss due to the attack by lepidopteran insect pests is reduced
The use of chemical insecticides in the cotton field is minimised
Better quality cotton is produced
Gene therapy has been successful in curing Codes genetic diseases in laboratory animals through
exposure to X-ray to rectify the defective gene
replacing the defective gene with a functional gene
oral delivery of genes
use of therapeutic medicines to rectify the defective gene
The replacement of a normal gene by its specifically mutated copy is
Gene cloning
Gynogenesis
Gene knockout
lnterparietal hybridisation
A natural form of genetic engineering is exhibited by
Haemophilus influenzae
E.coli
Dolly
Agrobacterium tumefaciens