Explain the role(s) of the following in biotechnology.(a) Restri

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 Multiple Choice QuestionsLong Answer Type

101.

Give reasons why:
a. Most zygotes in angiosperms divide only after certain amount of endosperm is formed.

b. Groundnut seeds are exalbuminous and castor seeds are albuminous.

c. Micropyle remains as a small pore in the seed coat of a seed.

d. Integuments of an ovule harden and the water content is highly reduced, as the seed matures.

e. Apple and cashew are not called true fruits.

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 Multiple Choice QuestionsShort Answer Type

102.

Plenty of algal bloom is observed in a pond in your locality.
(a) Write what has caused this bloom and how does it affect the quality of water.
(b) Suggest a preventive measure.

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103.

(a) List the three states the annuals and biennial angiosperms have to pass through during their life cycle.
(b) List and describe any two vegetative propagules in flowering plants.

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104.

Describe how do ‘flocs’ and ‘activated sludge’ help in sewage treatment.

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105.

Explain the role(s) of the following in biotechnology.
(a) Restriction endonuclease
(b) Gel-electrophoresis
(c) Selectable markers in pBR322


(a) Restriction Endonucleases These are the bacterial enzymes that cut dsDNA into fragments after recognising and binding to the specific nucleotide sequences, known as recognition site. These enzymes are used to form recombinant molecules of DNA, composed of DNA from different sources.

(b) Gel-Electrophoresis is the technique which allows the visualisation of separated fragments of DNA on an agarose gel matrix.
Since, the DNA fragments are negatively charged molecules, they separate and move towards the anode (+ ve) under the influence of an electric field. DNA fragments are separated on the basis of their size through the sieving effect provided by the gel.

(c) Selectable Markers in pBR322, an E. coli cloning vector has two antibiotic resistance genes, i.e. for ampicillin and tetracycline. Antibiotic resistance is used as selectable marker, i.e. it helps in identification and selection of transformants. For example, when a foreign DNA is ligated at the site of tetracycline resistance (tet r ,) gene in pBR322, the recombinant plasmid will lose tetracycline resistance due to insertional inactivation of foreign DNA, but can still be selected out from non-recombinants by placing the transformants on ampicillin containing medium. The transformants growing on ampicillin containing medium, are then transferred on tetracycline containing medium. The recombinants will grow on ampicillin containing medium but not on tetracycline one whereas non-recombinants grow on both.

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 Multiple Choice QuestionsLong Answer Type

106.

Public all over India is very much concerned about the deteriorating air quality in large parts of North India. Alarmed by this situation the Resident’s Welfare Association of your locality organised an awareness programme entitled ‘Bury not burn’. They invited you, being a biology student to participate.
(a) How would you justify your arguments that promote burying and discourage burning?
(Give two reasons)
(b) With the help of flow charts, one for each practice, depict the chain of events that follow.

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 Multiple Choice QuestionsShort Answer Type

107.

List four benefits to human life by eliminating the use of CFCs .

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108.

Secondary treatment of the sewage is also called Biological treatment. Justify this statement and explain the process

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109.

How did a citizen group called Friends of Arcata Marsh, Arcata, California, USA, help to improve water quality of the marshland using Integrated Waste Water Treatment ? Explain in four steps.


110.

Looking at the deteriorating air quality because of air pollution in many cities of the country, the citizens are very much worried and concerned about their health. The doctors have declared a health emergency in the cities where the air quality is very severely poor.

(a) Mention any two major causes of air pollution.

(b) Write the two harmful effects of air pollution on plants and humans.

(c) As a captain of your school Eco-club, suggest any two programmes you would plan to organise in the school so as to bring awareness among the students on how to check air pollution in and around the school


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