PCR and Restriction Fragment Length Polymorphism are the methods

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 Multiple Choice QuestionsMultiple Choice Questions

171.

Which one of the following a wrongly matched?

  • Transcription - Writing information from DNA to t-RNA

  • Translation - Using information in m - RNA to make protein

  • Repressor protein - Binds to operator to stop enzyme synthesis

  • Repressor protein - Binds to operator to stop enzyme synthesis

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172.

Transformation was discovered by 

  • Meselson and Stahi

  • Hershey and Chase

  • Griffith

  • Griffith

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173.

The first human hormone produced by recombinant DNA technology is 

  • insulin

  • estrogen

  • thyroxin

  • thyroxin

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174.

An analysis of chromosomal DNA using the Southern hybridization technique does not use

  • electrophoresis

  • blotting

  • autoradiography

  • autoradiography

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175.

Removal of RNA polymerase -III from nucleoplasm will affect the synthesis of

  • tRNA

  • rRNA

  • mRNA

  • mRNA

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176.

PCR and Restriction Fragment Length Polymorphism are the methods for

  • Study of enzymes

  • Genetic transformation

  • DNA sequencing

  • DNA sequencing


D.

DNA sequencing

The hypervariable regions of DNA have Variable Number of Tandem repeats (VNTRs) of short nucleotide sequences which are specific for each individual (except for monozygotic twins). Variations also occur due to mutations, small deletions or insertions. Therefore, DNA cut by a particular endonuclease will have different lengths in different persons. This phenomenon is called Restriction Fragment Length Polymorphism. It is the basis of genetic (Or DNA) fingerprinting and is useful in identifying individuals from their semen, blood or tissues or any other DNA sample and resolution of parent-hood disputes. 
Polymerase Chain Reaction (PCR) is also useful in genetic fingerprinting as it can amplify the DNA sample even if available in a very small amount

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177.

Which one of the following is not a part of a transcription unit in DNA?

  • The inducer

  • A terminator

  • A promoter

  • A promoter

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178.

A single strand of nucleic acid tagged with a radioactive molecule is called

  • vector

  • selectable marker

  • plasmid

  • plasmid

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179.

Ribosomal RNA is actively synthesised in 

  • lysosomes

  • nucleolus

  • nucleoplasm

  • nucleoplasm

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180.
Which one is a true statement regarding DNA polymerase used in PCR?
  • It is used to ligate introduced DNA in recipient cells

  • It serves as a selectable marker

  • It is isolated from a virus

  • It is isolated from a virus

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