Some of the steps of DNA fingerprinting are given below. Identify

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 Multiple Choice QuestionsMultiple Choice Questions

301.

DNA replication generally proceeds in a 

  • 3 direction

  • 5 direction

  • 3 direction

  • 5 direction


302.

DNA polymerase enzyme is required for synthesis of

  • DNA from RNA

  • RNA from DNA

  • DNA from DNA

  • RNA from RNA


303.

Which type of DNA is found in bacteria

  • Helical DNA

  • Membrane bound DNA

  • Straight DNA

  • Circular free DNA


304.

The chemical knifes of DNA are

  • polymerases

  • ligases

  • endonucleases

  • amylases


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305.

Refer to the given figure and select the correct option regarding its parts labelled as A, B and C.

  • A-Histone octamer, C-DNA

  • B-H1 histone, C-Histone octamer

  • A-H1 histone, B-DNA

  • A-Histone octamer, C-H1 histone


306.

Match the triplet codons listed in column I with their amino acids mentioned in column II and select the correct option from the given codes.
          Column I             Column II
        A. GGG                    (i) Proline
        B. UUU                    (ii) Glycine
        C. CCC                    (iii) Arginine
        D. AAA                    (iv) Phenylalanine
                                      (v) Lysine

  • A-(v), B-(iii), C-(i), D-(ii)

  • A-(v), B-(iii), C-(i), D-(iv)

  • A-(ii), B-(iv), C-(i), D-(v)

  • A-(i), B-(ii), C-(iii),D-(iv)


307.

Radio-tracer technique shows that DNA is in

  • single-helix stage

  • double-helix stage

  • multi-helix stage

  • none of these


308.

Assertion: The honeybee queen copulates only once in her life time.

Reason: The honeybee queen can lay fertilised as well as unfertilised eggs.

  • If both assertion and reason are true and reason is the correct explanation of assertion.

  • If both assertion and reason are true but reason is not the correct explanation of assertion.

  • If assertion is true but reason is false.

  • If both assertion and reason are false.


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309.

In a 3.2 Kbp long piece of DNA, 820 adenine bases were found. What would be the number of cytosine bases?

  • 780

  • 1560

  • 740

  • 1480


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310.

Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the options given.

A. Electrophoresis of DNA fragments
B. Hybridisation with DNA probe
C. Digestion of DNA by restriction endonucleases
D. Autoradiography
E. Blotting of DNA fragmentsto nitrocellulose membrane

  • C - A - B - E - D

  • C - A - E - B - D

  • A - E - C - B - D

  • A - C - E - D - B


B.

C - A - E - B - D

The technique of DNA fingerprinting involves following steps:

(i) Isolation of DNA from sample cell

(ii) Amplification of DNA using Polymerase Chain Reaction (PCR), (if DNA is less in amount).

(iii) Digestion of this DNA by restriction endonucleases (C)

(iv) These DNA fragments are electrophoresed in agarose gel. These fragments can be visualised using EtBr - UV system (A)

(v) Separated DNA fragments are transferred to nitrocellulose membrane using Southern Blotting (E).

(vi) Probing for VNTRs is done using labelled DNA probes (B).

(vii) The hybridised fragment can be detected by autoradiography (D).


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