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Biotechnology : Principles and Processes

Multiple Choice Questions

41.

In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is

Indian Council of Medical Research (ICMR)
Council for Scientific and Industrial Research (CSIR)
Genetic Engineering Appraisal Committee (GEAC)
Research Committee on Genetic Manipulation (RCGM)

42.

The correct order of steps in Polymerase Chain Reaction (PCR) is

Extension, Denaturation, Annealing
Annealing, Extension, Denaturation
Denaturation, Annealing, Extension
Denaturation, Extension, Annealing

43.

All monerons

Contain DNA and RNA
Demonstrate a long circular strand of DNA, not formed enclosed in a nuclear membrane
Are bacteria
All of the above

44.

Skin colour is controlled b

Single gene
3 pairs of genes
2 pairs of genes
2 pairs of genes with an intragene

45.

Gyms revoluta is popularly known as

Sago palm
Royal palm
Date palm
Sea palm

46.

In DNA, when AGCT occurs, their association is as per which of the following pair

A-G, C-T
A-T, G-C
A-C, G-T, A-C, E-T
All of these

47.

A segment of DNA has 120 adenine and 120 cytosine bases. The total number of nucleotides present in the segment is

48.
Gel electrophoresis is used for

Cutting of DNA into fragments

Separation of DNA fragments according to their size

Construction of recombinant DNA by joining with cloning vectors

Isolation of DNA molecule

Separation of DNA fragments according to their size

When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves the movement of fragments or molecules on the basis if molecule size and shape under the influence of electric field.

Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. It cleaves DNA only within or near recognition sites, which have specific base sequences, such endonucleases are known as a restriction endonucleases.

The selected vector is cut open with a restriction enzyme that has a unique target site located in the sequence, where the DNA insert should be integrated to facilitate the selection of recombinant DNA.