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The term genetic engineering is used for :

  • blotting technique

  • RNA reaction technique

  • protein synthesis technique

  • recombinant technique


recombinant technique

Recombinant DNA technology is used as a synonym for DNA cloning or gene cloning. It is also known as genetic engineering.


Micro-organism which act as a vehicle for foreign gene transfer in higher plants

  • Agrobacterium

  • E. coli

  • T.M.V.

  • Bacillus anihracis



The bacterium-Agrobacterium acts as a vehicle for transfer of DNA or genes from one organism to another organism because it infects all dicot plants.


Humulin is a :

  • form of chitin

  • human insulin

  • digestive enzyme

  • antibiotics


human insulin

Humulin is a human insulin produced by genetically engineered organisms.


The substrate for photorespiration is :

  • glycolate

  • acetyl Co-A

  • pyruvic acid

  • glucose



Photorespiration is a kind of respiration that occurs in photosynthetic cells only. In this process chloroplast, mitochondria and peroxisomes are involved. The substrate for photorespiration is glycolate formed in the chloroplast. It is converted enzymatically into glyoxalate in peroxisome which further changes into glycine. In mitochondria glycine changes into serine. It is the characteristic feature of C3 plants.


Which of the following pairs is correctly matched

  • Central dogma- Codon

  • Okazaki fragments - Splicing

  • RNA polymerase -RNA primer

  • Restriction enzymes - Genetic engineering


Restriction enzymes - Genetic engineering

Restriction enzyme recognises specific nucleotide sequences in DNA and then makes a double-stranded cleavage of DNA molecule. Restriction enzymes are called genetic scissors as they slice off genes and is recently being used in gene therapy technique. They are key tool of genetic engineering. Central dogma is related with protein synthesis. Okazaki fragments are found during DNA synthesis and RNA polymerase is used in synthesis of RNA.


Which is Indian dwarf wheat?

  • T. dicoccum

  • T. sphaerococcum

  • T. turgidum

  • T. aestivum


T. sphaerococcum

Triticum aestivum is an Indian dwarf wheat. It is endemic to Southern Pakistan and North- western India.


Cultivation of Bt cotton has been much in the news. The prefix Bt means

  • "Barium-treated" cotton seeds

  • "Bigger thread" variety of cotton with better tensile strength

  • produced by "biotechnology" using restriction enzymes and ligases 

  • carrying an endotoxin gene from Bacillus thuringienesis.


carrying an endotoxin gene from Bacillus thuringienesis.

Some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans (tobacco budworm, armyworm), coleopterans (beetles) and dipterans (flies, mosquitoes). B. thuringiensis forms protein crystals during a particular phase of their growth. These crystals contain a toxic insecticidal protein.


Assertion : "Rosie", the first transgenic cow, produced human protein-enriched milk.

Reason : The milk contained the human alpha­ for human babies than natural cow milk.

  • If both assertion and reason are true and reason is the correct explanation of assertion.

  • If both the assertion and reason are true but reason is not the correct explanation of assertion.

  • If assertion is true but reason is false.

  • If both the assertion and reason are false.


If both assertion and reason are true and reason is the correct explanation of assertion.

In 1997, the first transgenic cow, Rosie, produced human protein-enriched milk at 2.4 grams per litre. This transgenic milk is a more nutritionally balanced product than natural bovine milk and could be given to babies or the elderly with special nutritional or digestive needs.


Genetically engineered human insulin is called

  • humulin

  • haematin

  • hybridoma

  • hybrid



In 1983, an American company Eli-Lilly, produced the first genetically engineered human insulin often called as humulin.


Restriction enzymes are used in genetic engineering, because they

  • can degrade harmful proteins

  • can join different DNA fragments

  • can cut DNA at specific base sequence

  • are nucleases that cut DNA at variable sites


can cut DNA at specific base sequence

Restriction enzyme are also known as molecular knifes or molecular scissors. They are isolated from bacteria. They recognize specific base sequence in DNA and cleave both strands of it.